Stable Transfection:Article Title: Targeting the human MUC1-C oncoprotein with an antibody-drug conjugate
Article Snippet: .. Human HCT116 colon cancer cells (ATCC) transduced to stably express an empty vector or MUC1 ( 22 ), human MDA-MB-468 breast cancer cells (ATCC) transduced to express a control scrambled CshRNA or a MUC1 shRNA ( 23 ), and mouse MC-38 colon cancer cells (provided by J. Schlom, National Cancer Institute, Bethesda, Maryland, USA) transduced to stably express an empty vector or MUC1 were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS), 100 μg/ml streptomycin, and 100 units/ml penicillin. .. Human HCC827 NSCLC, H441 NSCLC, and ZR-75-1 breast cancer cells (ATCC) were grown in RPMI1640 medium supplemented with HI-FBS, streptomycin, and penicillin.
Plasmid Preparation:Article Title: Targeting the human MUC1-C oncoprotein with an antibody-drug conjugate
Article Snippet: .. Human HCT116 colon cancer cells (ATCC) transduced to stably express an empty vector or MUC1 ( 22 ), human MDA-MB-468 breast cancer cells (ATCC) transduced to express a control scrambled CshRNA or a MUC1 shRNA ( 23 ), and mouse MC-38 colon cancer cells (provided by J. Schlom, National Cancer Institute, Bethesda, Maryland, USA) transduced to stably express an empty vector or MUC1 were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS), 100 μg/ml streptomycin, and 100 units/ml penicillin. .. Human HCC827 NSCLC, H441 NSCLC, and ZR-75-1 breast cancer cells (ATCC) were grown in RPMI1640 medium supplemented with HI-FBS, streptomycin, and penicillin.
Multiple Displacement Amplification:Article Title: Targeting the human MUC1-C oncoprotein with an antibody-drug conjugate
Article Snippet: .. Human HCT116 colon cancer cells (ATCC) transduced to stably express an empty vector or MUC1 ( 22 ), human MDA-MB-468 breast cancer cells (ATCC) transduced to express a control scrambled CshRNA or a MUC1 shRNA ( 23 ), and mouse MC-38 colon cancer cells (provided by J. Schlom, National Cancer Institute, Bethesda, Maryland, USA) transduced to stably express an empty vector or MUC1 were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS), 100 μg/ml streptomycin, and 100 units/ml penicillin. .. Human HCC827 NSCLC, H441 NSCLC, and ZR-75-1 breast cancer cells (ATCC) were grown in RPMI1640 medium supplemented with HI-FBS, streptomycin, and penicillin.
Control:Article Title: Targeting the human MUC1-C oncoprotein with an antibody-drug conjugate
Article Snippet: .. Human HCT116 colon cancer cells (ATCC) transduced to stably express an empty vector or MUC1 ( 22 ), human MDA-MB-468 breast cancer cells (ATCC) transduced to express a control scrambled CshRNA or a MUC1 shRNA ( 23 ), and mouse MC-38 colon cancer cells (provided by J. Schlom, National Cancer Institute, Bethesda, Maryland, USA) transduced to stably express an empty vector or MUC1 were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS), 100 μg/ml streptomycin, and 100 units/ml penicillin. .. Human HCC827 NSCLC, H441 NSCLC, and ZR-75-1 breast cancer cells (ATCC) were grown in RPMI1640 medium supplemented with HI-FBS, streptomycin, and penicillin.
shRNA:Article Title: Targeting the human MUC1-C oncoprotein with an antibody-drug conjugate
Article Snippet: .. Human HCT116 colon cancer cells (ATCC) transduced to stably express an empty vector or MUC1 ( 22 ), human MDA-MB-468 breast cancer cells (ATCC) transduced to express a control scrambled CshRNA or a MUC1 shRNA ( 23 ), and mouse MC-38 colon cancer cells (provided by J. Schlom, National Cancer Institute, Bethesda, Maryland, USA) transduced to stably express an empty vector or MUC1 were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS), 100 μg/ml streptomycin, and 100 units/ml penicillin. .. Human HCC827 NSCLC, H441 NSCLC, and ZR-75-1 breast cancer cells (ATCC) were grown in RPMI1640 medium supplemented with HI-FBS, streptomycin, and penicillin.
Cell Culture:Article Title: Targeting the human MUC1-C oncoprotein with an antibody-drug conjugate
Article Snippet: .. Human HCT116 colon cancer cells (ATCC) transduced to stably express an empty vector or MUC1 ( 22 ), human MDA-MB-468 breast cancer cells (ATCC) transduced to express a control scrambled CshRNA or a MUC1 shRNA ( 23 ), and mouse MC-38 colon cancer cells (provided by J. Schlom, National Cancer Institute, Bethesda, Maryland, USA) transduced to stably express an empty vector or MUC1 were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS), 100 μg/ml streptomycin, and 100 units/ml penicillin. .. Human HCC827 NSCLC, H441 NSCLC, and ZR-75-1 breast cancer cells (ATCC) were grown in RPMI1640 medium supplemented with HI-FBS, streptomycin, and penicillin.
Modification:Article Title: Targeting the human MUC1-C oncoprotein with an antibody-drug conjugate
Article Snippet: .. Human HCT116 colon cancer cells (ATCC) transduced to stably express an empty vector or MUC1 ( 22 ), human MDA-MB-468 breast cancer cells (ATCC) transduced to express a control scrambled CshRNA or a MUC1 shRNA ( 23 ), and mouse MC-38 colon cancer cells (provided by J. Schlom, National Cancer Institute, Bethesda, Maryland, USA) transduced to stably express an empty vector or MUC1 were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS), 100 μg/ml streptomycin, and 100 units/ml penicillin. .. Human HCC827 NSCLC, H441 NSCLC, and ZR-75-1 breast cancer cells (ATCC) were grown in RPMI1640 medium supplemented with HI-FBS, streptomycin, and penicillin.
Fluorescence:Article Title: Tubulin inhibitory activity of a novel colchicine-binding compounds based on a dinaphthospiropyranran scaffold.
Article Snippet: Spiropyrans have been investigated for their thermoand photochromic characteristics, but their biotherapeutic properties have not been addressed.. We report anti-proliferative properties of a novel dinaphthospiropyran analogue (1).. The compound 1 was synthesized by a simple and expedient method using a one-pot acid-catalyzed aldol condensation of 2-hydroxy-1-naphthaldehyde with 4-piperidone followed by an acetalization reaction.
Microscopy:Article Title: Tubulin inhibitory activity of a novel colchicine-binding compounds based on a dinaphthospiropyranran scaffold.
Article Snippet: Spiropyrans have been investigated for their thermoand photochromic characteristics, but their biotherapeutic properties have not been addressed.. We report anti-proliferative properties of a novel dinaphthospiropyran analogue (1).. The compound 1 was synthesized by a simple and expedient method using a one-pot acid-catalyzed aldol condensation of 2-hydroxy-1-naphthaldehyde with 4-piperidone followed by an acetalization reaction.
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